The Research Of MtDNA Genetic Polymorphism In Dong-Guan Han Population

Objective: To amplify the mtDNA gene pool of Guang-Dong Han Populations which is a ancient population in Guang-Dong Province, the research the mtDNAs of the Dong-Guan Han Population and analysis the mtDNA haplogroups have been done. Comparing the mtDNA gene pool with other region Han Populations and ethnic Populations to know the Guang-Dong Han Population origins, evolving, migration and the genetic characters.Methods: Blood samples of 107 unrelated individuals from Dong-Guan Han Population were collected with appropriate informed consent. Genomic DNA was extracted by. Sequencing the HVSâ… ,â…¡to find the mutation sites in the HVSâ… ,â…¡by comparing with the CRS. Haplotyping the mtDNAs by the mutation sites and other data include the RFLP etc. The genetic distance and principal component analysis have been done with these mtDNA data.Results: Blood samples of 107 unrelated individuals from Dong-Guan Han Population were collected with appropriate informed consent. The mtDNA mutation sites in HVSâ… ,â…¡and coding region have been funded. There are some (sub-) haplogroups existing in the Dong-Guan Han Population. Haploroup D, M7, B and F1 are existed with high frequency. These haplogroups including A, C, G2 and Z have a relative low frequency. By comparing the other regional Han Populations, Dong-Guan Han's maternal genetic construction is similar with the Guang-Zhou Han and Yun-Nan Han Populations. At the same time Dong-Guan Han Population have a proximate genetic relationship between the Yao, Miao and Dai ethnic Groups.Conclusion: The Guang-Dong Han Populations have a typical south origin mtDNA haplogroup profile by analysis the Han Populations. The maternal genetic structure of Guang-Dong Han Population can be representing by the Dong-Guan Han Population. The Guang-Dong Han population have originate from Bai-Yuei Tribe and gene admixture with other ethic populations, such as Miao populations, Yao populations..