Effects Of V1a Receptor To Social Behaviors And Expression Of AR Of Male Mandarin Voles Microtus Mandarins

Neural basis of social behavior has been become one of focus in behavioral neuroscience. Social behaviors conclude a series of social interaction following individual encountering each other in the same population. Animal social behavior, was controlled and regulated by centre nervous system and neuroendocrine system. Neuropeptide, sex hormone, a number of endocrine factors and environments where animal lives cooperate to affect social behavior of animals. More recently, neuropeptide AVP have been found to be related closely with social organization in voles and social behaviors. AVP could affect many social behaviors, such as social recognition, aggression, sexual behavior, partner preference, affinity behavior and individual behavior. Besides, AR also participated in modulating animal social behavior. So, the present study will aim at the two problems: 1, how AVP could affect some social behaviors in voles; 2, Is there possibility of effects to AR expression by AVP receptor?Because the male voles could have abundance of AVP, Androgen and its receptor, we used monogamous male mandarin voles (Microtus mandarinus) as research objects. Combining antisense nucleotides tools with behavioral examination, we examined the correlative behavior after i.c.v, continuous injection of antisense nucleotides of AVP V1a receptor. Meanwhile, Combined antisense nucleotides tools with in situ hybridization, we discussed the problem how AVP, Androgen and its receptor controlled complex social behaviors in male voles.1. Selected male adult mandarin voles were given i.c.v, continuous injections of antisense nucleotides of AVP V1a receptor respectively in first day, third day and fifth day. After six hours, we examined aggression behavior during 10 minutes: The results were following: Compared with group of injection of scramble nucleotides, anrisense group showed significant reduction in frequency and total duration in aggression behavior. We suggested that antisense nucleotides of AVP V1a receptor inhibit the translation of V1aR in mRNA levels. Furthermore, it blocked AVP to modulate aggression behavior in male voles.2. Selected male adult mandarin voles were given i.c.v, continuous injections of antisense nucleotides of AVP V1a receptor in first day, third day and fifth day. After six hours, we examined partner preference behavior during 60 minutes. The results were following: There were significant differences not only in visiting duration but also in aggression, defensive behavior, investigating behavior, amicable behavior, reproductive behavior and nonsocial behavior. It is suggested that continuous injection of antisense nucleotides of AVP V1a receptor can suppress social recognition after i.c.v, continuous injection of antisense nucleotides of AVP V1a receptor in male adult voles and reduced the pair bond formation of male and female voles.3. Selected male adult mandarin voles were given i.c.v, continuous injections of antisense nucleotides of AVP V1a receptor in first day, third day and fifth day. After six hours, we examined affinity behavior during 60 minutes. The results were following: There were significant differences not only in visiting duration, but also in defensive behavior, investigating behavior, amicable behavior, and nonsocial behavior. It suggested that there were different nervous circuit, which male voles recognized socially its sibling and stranger voles in the preference behavior examination.4. Selected male adult mandarin voles were given i.c.v, continuous injections of antisense nucleotides of AVP V1a receptor in first day, third day and fifth day. After six hours, we examined individual behavior during 30 minutes. The results were following: There were significant differences in aggression, defensive behavior and amicable behavior but not in investigating behavior, reproductive behavior and nonsocial behavior. There were the two opposite manners about defensive behavior and amicable behavior, but there were similar changing tendency in the behavior examination. It might show us that there were different roles in consideration of different behavior examinations while antisense nucleotides of AVP V1a receptor affected behaviors of male mandarin voles. What's more, it also suggested us that there were different mechanisms and environment factors on defensive behavior and amicable behavior.5. Selected male adult mandarin voles were given i.c.v, continuous injections of antisense nucleotides of AVP V1a receptor in first day, third day and fifth day. After six hours, AR mRNA was examnined using in situ hybridization method in male voles. The results were following: There were significant differences in the LS, BNST, AH, ME and VMH. Compared with injection of scramble nucleotides, there were significant reduction in the number of AR mRNA in LS, AH, ME and VMH. Compared with injection of saline, there were significant reductions in the number of AR in these nucleuses. These data showed that the roles between antisense nucleotides of AVP V1a receptor and AR affected social behavior in the male voles. It explained that AVP V1a receptor modulated social behavior in male voles possibly through Androgen and its receptor.