The Cloning And Bioinformatics Analysis Of The CDNA Of Oat β-1, 3-glucanase Gene

Primers were designed on the basis ofβ–1,3–glucanase′s conservative sequence in Genebank, The completed sequence ofβ–1,3–glucanase gene in oat(Oglc13) was acquired by RT-PCR and RACE. Preliminary analysis by Internet data and bioinformatics tools indicated that: The completed sequence was composed by 1191bp, including a completed ORF which is composed of 1008bp; TGA is termination condon, 5′noncoding region which was composed by 49bp lies in initiation codon′s upstream; 3′noncoding region which was composed by 121bp lies in termination codon′s downstream; it includes a signal added poly(A) and a tail which is composed of 13 adenosine monophosphate .The homology ofβ–1,3–glucanase amino acids sequence is 70%～77% compared oat with enunciable barely,wheat,rice and rye; The protein encoded by Oglc13 gene is a kind of hydrophobous transmembrane protein, it′s relative molecular weight is 35479.3 Da, isoelectric point is 8.81, it contains Ala,Val and Ary redundantly ; its secondary structure contain 12α-helixes, 22 aperiodical coils; it contain a Cosy_Hydrol_F17 Glycosyl hydrolases family 17 signature; and it locates in vacnolus, The gene clone establish foundation in order to research the relations of Oglc13 gene′s structure,expression,regulation mechanism and function.