The Rapid Assaying Of IgG Content By The Staphycacol Protein A Labeled With The Colloidal Gold

IThe paper takes detecting the swine blood serum as an example through three parts:SPA labeled with colloidal gold preparation;Optimizing of the method condition;The evaluating experiment of method and applies primarily,establishes one method to detect the IgG content rapidly.SPA labeled with colloidal gold preparation.This experiment first uses the diameter 10nm colloid gold to label SPA(the golden yellow staphylococcus protein A),with the ultracentrifugation purified SPA -coated gold particles,also called the gold probe,and has carried on the examination to its biological activity and stability by two point method.The results showed that the obtained gold probe activeness is better,and it can be preserved stably for three months or so.Optimizing of the method condition.From four influencing factors of the gold probe density,the solution PH,the detection temperature and the response time to carry on the discussion,obtains the best detection condition is the best gold probe density is 1:4;The best PH is 7.4;In order to simplify the experiment,We decided to characterize the assaying performance at room temperature(±0.5℃);Reaction time about 40 minutes.Taking the swine blood serum IgG as the example,the best surem dilution multiple is 1:1000.The evaluating experiment of method and applies primarily.,then through the linear scope experiment,the duplicated experiment,the returns-ratio experiment,the method contrast experiment to evaluate this immunassay.And has carried separately on the examination with the newly built method to the blood serum samples of grow-up swine and the different date age newborn pig has confirmed furtherly that this method is feasibile and useful.The results showed that this method is highly sensitive,the lowest detection limits may reach 2.16μg/mL and the highest detection limits is 116.67μg/mL,the returns-ratio reaches 98.18%;in approves the coefficient of variation CV%=4.54%;among approves CV%=5.88%;Contrasted with the ELASA method,determination value between these two method has the highly correlational dependence,also r=0.9945,but this method operation is simple and fast. one step completed,it does not have to wash in many times,simplifying the automated operation.So it has very high clinical using value.