Effect Of Temperature On Chemiluminescence Of Luminol-H₂O₂ System And Determination Of Bovine Albumin

The luminescence is strongly influenced by chemical conditions in solution during the reaction of chemiluminescence. We can enhance the selectivity and sensitivity of luminescence through investigation on these factors, such as solvent,pH, surfactant and temperatures etc. This paper is studied on the relationship of between temperatures and chemiluminescence of luminol-H₂O₂ system.It is reported that chemiluminescence is mainly influenced by four factors, such as the solvent of reaction, the value of pH, surfactant and temperatures etc. Many researchers have studied on the former three factors, but the effect of temperature on chemiluminescence of luminol-H₂O₂ system is rarely reported. One is the chemiluminescence has poor repeat and difficult to measure; the other is the temperature of reaction is difficult to control and is easy disturbed by many factors at high temperatures. It is well known that when temperature is increasing and the CL would be added. In general, there are two ways to determine the effect of temperature on chemiluminescence: One is when temperature is added 1℃, the intensity of CL would be increased 2 percent; the second is the vant's Hoff Coefficient Q₁₀ =I_T+10/I_T. Whereas, the two described are not clearly at effect of temperature on CL. So it is very important that we find a relationship between temperatures and intensity of chemiluminescence.The effects of different temperatures on luminol-H₂O₂ chemiluminescence (CL) were studied. A equation was proposed that it was linear between the logarithmic of CL of luminol-H₂O₂ with reciprocal of temperature: In I_max=-0.3392/T +6.0896, and correlation coefficient is 0.9926. At the experiments, we also study on the effect of temperature on mental ions -luminol-H₂O₂ system. When Cr³⁺ or Co²⁺ or Cu²⁺ is added in the luminol-H₂O₂ solution as catalyzes at different temperatures, the results indicate that the intensity of CL is enhanced at low temperature but we get opposite results at high temperatures. We can observe maximum CL at 40℃when Cr³⁺ or Co²⁺ is added, and Cu²⁺ is about 60℃. However,with the temperatures increasing and the CL is enhanced when Fe²⁺ or Mn²⁺ is added in the luminol-H₂O₂ solution. These results led us to the conclusion that the CL of luminol-H₂O₂ system has optimum conditions when different metal ions as catalyzes. So we can get the maximum CL to enhance the sensitivity at these optimum conditions.On the other hand, we have researched the mechanism on the CL of mental ions-luminol-H₂O₂ system. Firstly mental ions form a 6-membered ring with luminol and then the chelate is oxidized by H₂O₂ to the intermediate of nitrine quinone compound; secondly it is oxidized to the excited aminophthalate ion and eradiated the light.In addition, Using phthalodinitrile and zinc sulfate as starting materials, we synthesize Zinc Phthalocyanine (ZnPc) under microwave irradiation. After sulfonation of ZnPc, the product becomes ZnPcSn(n=1～4), and it possesses enhanced Resonance Rayleigh Scattering (RRS) caused by bovine albumin (BA).There is a RRS peak at 470nm,and the enhanced intensity of BA at this wavelength is proportional to the concentration of BA. So a simple and rapid method of RRS for the determination of BA is established. The linear range is 0.25～20μg/mL. The detection limit for BA is 0.17μg/mL and the method has good selectivity.