| PA1b is a single chain multifunction peptide, consisted of 37 amino acid residues with 6 cysteines at 3, 7, 15, 20, 22 and 32 of chain positions,and its molecular weight is 3,742Da. This peptide is involved in a growth or metabolic regulation mechanism in a similar fashion to insulin and insulin-like growth factors. It can bind basic 7S globulin(7S Bg) which exsisted in soybean seeds, and 7S Bg also can bind insulin and insulin-like growth factors, so it named leginsulin. NMR study showed that PA1b and insulin possess similar tertiary structure, which did Bg and insulin receptor also. So we are interesting in the study whether PA1b can regulate the blood sugar concentration similar insulin. Especially, this plant peptide hormone was isolated in pig intestin, we also interesting in whether PA1b is a new incretin-like peptide hormone .In this study, PA1b and its isoforms had been isolated from pea seeds and characterized, then the method for largly preparation PA1b was established; It had been proved that the resist-pepsin-degradation activity of PA1b was related to its Cystine knot motif. Subcutaneous and intravenous injection was taken on normal mice model to investigate the influence of PA1b on blood sugar concentration , the influence of PA1b on blood sugar concentration was found and reasonable explanations were given, which provide the new thread for further investigation and application of this peptide.
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