Studies On The Interaction Of Protein With Azo Dyes By Resonance Light Scattering Spectra And Their Analytical Application

The interaction of protein with azo dyes was studied in this paper by resonance light scattering (RLS) technique and UV-vis spectra. It is very important to perfect determination of proteins. Four new methods for the determination of proteins based on the enhancement of RLS signals have been developed, It was found that the linear ranges and detection limits of BSA were 0.2-6.0 ng·mL^-1 and 0.9 ng·mL(-1) for Marshall red system, 0.01-5.0μg·mL(-1) and 1.4 ng·mL(-1) for Thiazine red R system, 0.15-3.0 ng·mL(-1)and 1.25 ng-mL(-1) for Diamine blue 3R system, 0.4-3.0μg·mL(-1) and 1.65 ng-mL(-1) for Diamine green B system It has been applied to the determination of BSA in synthetic samples with satisfactory results.