Identification Of Bmcyp306a1 And Bmbcl-2 Gene And Its Involvement In Fluoride-Resistance

In this research, the fluoride-resistent strain and the sensitive strain were used to identify genes linked to silkworm resistance to fluoride by the fluorescent differential display technique. The mechanism of fluoride was studied to provide the new knowledge on desiging related medicine to solve serious loss in sericicultural industry and protect human and other living thing from fluoride.The research process and the main conclusions are presented as the following:1. The fluorescent different display technique-PCR (FDD-PCR) was used to analyze differentially expressed genes in fluoride-resistant silkworm Bombyx mori strain 441 and susceptible strain 440, and a differentially expressed gene, Bmcyp306a1, was obtained. Q-PCR analyzed that Bmcyp306a1 had higher expression level in midgut of strain 441 than 440, which indicated that Bmcyp306a1 may be involved in fluoride resistance by high expression in tissues.2. Meanwhile, another highly expressed gene, Bmbcl-2, in resistant strain was obtained. RT-PCR results showed that when silkworm was treated with water, Bmbcl-2 expressed in five tissues such as midgut, fat body, lipid ovary and silkgland of strain 441DZ and 440DZ, and its expression level was also higher in 441DZ than that in 440DZ. When silkworm was treated with fluoride, the expression level of Bmbcl-2 was higher in 441F than that in 440F. These results indicated that the high expression level of Bmbcl-2 in resistant stain may be related with fluoride-resistance.