Dune Reed Unique Compounds Contained In The Membrane Of The Impact Of Mobility

Membrane is playing a cruical role in substance transportation between outside and inside of cell, maintain ion balance, information transmission, cell identification and cell movement and also other impotant life process.Using etiolated hypocotyledonary axis of mung bean and swamp reed as materials, 8-anilinonaphthalene-l-sulfonic and 1,6-diphenyl-1,3,5-hexatriene as probes, a fluorescence spectrophotometry method was performed to research the change of membrane fluidity of mitochondia treated by extraneous PAAC. Two factors will change the fluorescence of ANS when ANS combine with membrane. One is the polarity of ANS's environment changes, the other is the fluidity of membrane lipoid combined with ANS changes. If the first is invariable, that the intensity of ANS's fluorescence can reflect the fluidity of membrane lipoid. Fluorescence more intensity, slower of fluidity. And this fluorescence reflects the average value of all the lipoid region's movement. DPH is a sensitivity and commom probe when learn about the fluidity of membrane lipoid. When DPH mixes into the hydrocarbon chain region of membrane fat acid, medium will be more sticky and the transform of cis isomer will be suppressed, so that all are resver, that is can launch fluoresence. Owing to the long axis of DPH molecule is nearly paralleling with the long axis of membrane lipiod acid molecule. Therefore the degree of fluoresence polarization(P) can welly show the min-viscosity of membrane lipoid region, and quantificationally illuminate the movement of membrane lipoid molecule.The results of the study suggest that membrane fluidity of mitochondia have been reduced after treated by PAAC, furthermore, there is a dose-effect relationship. Confined within our experiment, PAAC reduces the membrane fluidity under the high temperature stress, more importantly, it can stabilize the fluidity of membrance, however, it has no effect on the change of the transition temperature. Therefore, it can be suppose that PAAC can stabilize and protect the function of the biomembrane in plants by reduceing the fludity under the high temprature in summer.