Study On Effects Of Chitosan And Its Derivatives On Lipoprotein

In this paper, several kinds of chitosan derivatives (O-CMCs, N-MPCs, N-C16,18QCs and O-C18OACs) with different molecular weights (6×103, 1×104, 3×104, 5×104, 1×105 and 1.5×105) were synthesized and effect of active groups of chitosan derivatives on solubility and electric charge characters were discussed. SDS-PAGE, blood adsorption and circular dichroism (CD) spectrum were used to analyse the effects of chitosan and its derivatives on Total cholesterol(TC)High density lipoprotein(HDL), Low density lipoprotein(LDL) and Triglyceride (TG). Water-soluble chitosan with 1×104 molecular weight ( M ), marked by Fluorescein iso-thiocyanate (FITC), was used for Pharmcokinetics(PK) study. The Blood and dung PK of SD mouse, feeding by FITC-Cs complexes solution, were researched through detecting the change of fluorescence intensity. FITC-Cs in many visceras were also detected after experiment.The results show that low molecular weight ( M <6×103) chitsan can adsorb LDL and Triglyceride (TG) obviously, but almost can't adsorb HDL. O-C18OACs ( M <1×104) has the same LDL selectivity. Water-soluble chitosan derivatives have more lipoprotein adsorption, as TC/ HDL and LDL/ HDL are reduced and higher molecular weight ( M <1×105) water-soluble chitosan derivatives may obtain the selective adsorption. The water-soluble chitosans especially N-C16,18QCs, effect theα-helix of HDL with hydrophilic surface (40.8%decline), but oil-soluble chitosan O-C18OACs effects theβ-sheet of LDL obviously.Pharmcokinetics(PK)result shows that the marked chitosan( M <1×104)metabolizes slowly(tmax=12h,τ1/2=48h), and only smaller chitosan can enter into blood and visceras, most other bigger ones may effect in alimentary canal, and finally drainaged with dung.