Lipidomics Analysis Of Differences Between Phospholipid Metabolism In Two Taxus Cell Lines

In order to elucidate the difference of phospholipid metabolism in two Taxus species,Taxus cuspidata and Taxus chinensis, we employ the lipidomics strategy to systematically study the phospholipids in the two species by HPLC/ESI/ MSn. The achievements in our study are as follows: (1) We establish a method of high throughput, high sensitivity and high discerning capacity to analyze the phospholipid profiles in Taxus cells. (2) We found that there are over 84 molecular species of 6 different phospholipids classes in Taxus cells, and we establish a Taxus phospholipid database basing on these results. A new discovery in this study was that the phospholipids in Taxus cells contain specific unsaturated long carbon chains, such as C20:3 and C20:4. (3) We measured the changes of phospholipid contents in normally cultured Taxus cuspidata and the automatically apoptotic Taxus chinensis. Through principal analysis of the main 77 species of phospholipids, the normally cultured Taxus cuspidata and the automatically apoptotic Taxus chinensis could be evidently distinguished. And the two negative correlating species on the first principal component-PC and PA mainly contributing to the distinguishment, and thus they may be the important biomarkers and their abnormal metabolism may play key roles in the automatic apoptosis of Taxus chinensis.In conlusion, we presume that the changes of phospholipid in Taxus chinensis are due to the activation of PLD(phospholipase D) and its subsequent degeneration of PC to PA, which finally lead to the automatic apoptosis. Our study establishes the foundation for further exploration of the phospholipid's roles on Taxus chinensis'apoptosis.