Research On Hardware Platform Of PCR

Polymerase Chain Reaction, which is called PCR for short, is a specific nucleic acid amplification technology in vitro. By this technology, researchers can choose to produce millions of copies of a specific DNA sequence from the complex DNA molecules groups, making the specific DNA segment amplified. The instrument that can automatically finish the polymerase chain reaction is called PCR instrument, whose full name is PCR Gene Amplification Instrument. Nowadays, PCR technology is widely used in molecular biology, medicine, law, biochemistry and other domains along with its development.This dissertation systematically discusses about the design of the hardware platform of PCR instrument. In addition, the test of temperature control algorithm is implemented based on the prototype machine. The main contents are as follows:The first chapter is a summary of the general situation of PCR technology. In this part I also explain the origin and significance of this studying theme and the main task of this thesis will also be put forward in this chapter.In chapter two I discuss in details about the power drive module of PCR instrument. Given the specialness of the Peltier Effect acting on the thermal electrical cooler(TEC), I contrive the detailed structure of the power drive module and illuminate the principles of setting corresponding parameters, enabling reliable, precise and bi-direction's direct current to supply the thermal electrical cooler.Chapter three is about the design of hardware platform for the temperature controller based on ARM embedded system. Besides, I also discuss about the design of high precision temperature data gathering module. With a focus on the temperature measurement and how to reduce the error, I finally settle down the high precision temperature measurement scheme with AD7711.In chapter four I study about temperature control algorithm and suggest a relay auto-tuning method and its application on temperature control system of PCR. The result seems to be fine.In chapter five I will do some experiments with the PCR prototype model and analyze the results. Actually this can be divided into two parts, one is testing on the power drive module and the other one is on the temperature control module.The last chapter is a conclusion of the whole thesis and there are also some perspective opinions about the posterior tasks in it.