Interferon α-2a In Liquid Chromatography Purification Of The Research

Interferon (IFN) secreted by human cells of a class of proteins. It has anti-virus, anti-tumor and immunomodulatory activity. Research experience of its four stages in the international arena in recent years, anti-virus and anti-cancer research, the interferon as a natural anti-viral protein in human attention more. China ratified in 1992 the production of genetically engineered interferon, is the first drug to market gene. In the large-scale production of interferon liquid chromatography purification method of production and have a great impact on the recovery rate, to improve the purity of interferon to reduce the pyrogen to enhance the quality is also important. The purpose of this study is to look at different methods of liquid chromatography to increase the purity of interferon to reduce the pyrogen to enhance the quality. Therefore, the application of four kinds of different chromatography methods, the same group of the same number of recombinant interferonα-2a samples of crude extract purification and the purity after purification, yield and comparison of Pyrogen in order to build a high-purity, high yield , the original low-heat method of purification in order to adapt to large-scale production, increase output. The liquid chromatography from different experimental methods, the exploration of a low-cost, high recovery rate of the purification line.