| Immunoassay with electrochemical detection is an attractive method due to its high sensitivity and low detection limit. It can also compensate its deficiency in differentiation with immunity. So it is of interest because of wide detection range and low detection limit. It is suitable for the analysis of small amounts, the instruments are simple, and the operation is convenient. So the linear sweep voltammetric was used to study the 2-amino-3- hydroxypyridine -H2O2 - horseradishperoxidase (HRP) voltammetric enzyme - linked immunoassay new system. So far, the systems were not repoted by others. The optimal condition of enzyme-catalyzed reaction were investigated, and the best reaction condition of these systems were selected. In addition, the virus clinico detection was also performed. The 2-amino-3- hydroxypyridine -H2O2-horseradish peroxidase (HRP) voltammetric enzyme-linked immunoassay new system had extremely high sensitivity. HRP can be measured with a detection limit of 1.2×10-13 g/mL and a linear range of 4.0×10-13～1.0×10-9 g/mL. The method was also used to detect AFP, PSA,CEA,T4,HCG,.This method has been successfully applied to the detection of alpha-fetoprotein (AFP). The detection limits was 0.1 ng/mL, and the linear range is 0.1～200 ng/mL. Under the optimum conditions, the human serum samples were detected. The results showed good corresponding relationship with those in spectrophotometric ELISA method. This method has been successfully applied to the detection of prostatespecific antigen (PSA). The detection limits was 0.10 ng/mL, and the linear range is 0.10 100.0 ng/mL. Under the optimum conditions, the human serum samples were detected. The results showed good corresponding relationship with those in spectrophotometric ELISA method.This method has been successfully applied to the detection of carcinoembryonic antigen (CEA). The detection limits was 0.10 ng/mL, and the linear range is 0.10 80.0 ng/mL. Under the optimum conditions, the human serum samples were detected. The results showed good corresponding relationship with those in spectrophotometric ELISA method.This method has been successfully applied to the detection of thyroxine (T4). The detection limits was 0.05μg/dL, and linear range is 0.05 30.0μg/dL. Under the optimum conditions, the human serum samples were detected. The results showed good corresponding relationship with those in spectrophotometric ELISA method.This method has been successfully applied to the detection of human chorionic gonagotropin (HCG). The detection limits was 1 ng/mL, and linear range is 1 400.0 ng/mL. Under the optimum conditions, the human serum samples were detected. The results showed good corresponding relationship with those in spectrophotometric ELISA method.
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