Characterization Of The Cold-adapted ATPase DNA From The Psychrophilic Ciliate Euplotes Focardii

Owing to the stringent request of the biocatalyst during the process of the industry produce, people had been seeking to finding out every kinds of kinase these years.Comparing with the synthetical organic,the biocatalyst has better characteristic in specific-selection.It could be used to product objective-production effectively and relieve the stress of the environment,although, until now it has been found more than 3000 kinds of enzymes and many of them have been already applied industry,the requirements of them still not been satisfied,the basic reason is the kinase could not undergo some type of the reaction condition.Viewing to this point,the microorganism of which exists in the extreme condition play a more and more important role in nowadays,they are precious new type resource in this field.The enzymes from the psychrophilic organism family have been applied,partly because of the function what could cut down the energy expenditure during the industry elaboration.They are used wildly in detergent additives,textile industry,food industry,bioremediation and biocatalysis.For example,if we use it on the degreaser,it should be effective in decontamination under the low temperature,it is totally feasible for them to applied in the lavement vocation,also there is a very huge business potential to use the enzyme such as protease,psychrophilic protease,amylase,lipase and in furthering considering,not only this vocation but also the food processing will get the benefit from the utility of these psychrophilic enzymes.The typical characteristic of them is high catalytic in moderate temperature and stability in low temperature.The application of conserving in low temperature of these enzymes has already become the warm spots.Using the biotechnology to introduce the especial functional fragments of extremophiles, especially,the psychrophilic organisms into the normal organisms becomes the important point in researching.So it needs the knowledge on the psychrophilic organism.The ciliates is a kind of good test mode living,because it is easy to obtain,the way of experiment is feasible,its bio-cycle is short and qualify of gene-reforming,phenotype analysis,but until now the genome of The ciliates is still a blank.The experiment use the psychrophilic Euplotes focardii as the materials which had been found in Antarctic,during the process of the DNA bank-establishing,obtained a group of genes, aim at presuming the biological function through supposing amino acid sequences.Many kinds of kits were used to make guarantee that the quality and purity of desired DNA, for comprehending the mechanism of psychrophilic enzyme,furthering for making contribution to the industry production.