The Isolation Of Microsatellite Loci In Scotophilus Kuhlii And The Study Of Population Genetics In Cynopterus Sphinx

Chiroptera is the second largest order in mammal. Although they have a close relationship with human, there is still few report about them, especially the population genetic studies by molecular methods. Two bats were regarded as the object of study in our paper. We isolated microsatellite loci of one Microchiroptera and did preliminary population genetic research on other Megachiroptera.The Lesser yellow house bat, Scotophilus kuhlii (Chiroptera: Vespertilionidae) is an insectivorous bat species and mainly distributes in the south of China and South-east Asia. In order to obtain molecular marker to study the S. kuhlii's population structure and genetic diversity, we constructed an enriched DNA microsatellite library of S. kuhlii, and obtained eleven pairs of special polymorphism microsatellite primers, also tested on a population of 36 individuals from Hainan province. Hardy-Weinberg and no linkage disequilibrium were found between loci pairs according to GENEPOP3.4. These microsatellite loci can be a powerful molecular tool for population genetics of S. kuhlii.Cynopterus sphinx is mainly distributed in tropical and semi-tropical areas, which is one main representative of fruit bats in our country. The impact of destroyed environment and habitat fragmentation by human activities are being concerned by more and more researchers. In this study, we used 8 microsatellite loci published before to analyze population structure and genetic diversity of 7 populations of C. sphinx. The results showed: (1) Average allele of all populations was 10.2. Observed heterozygosity was the highest in Jiangmen (0.8418) and lowest in Beihai (0.7274). Two of all populations showed no significant departure from Hardy-Weinberg equilibrium and five populations appeared heterozygote deficiency. (2) The analysis of population differentiation showed that pairwise F_ST were significant differentiation(P< 0.001)except two population pairs. (3) A strong isolation by distance was indicated across all populations (b = 2E - 05, R² = 0.916, P < 0.001), but no similar support in Chinese populations. Construction of a dendrogram using UPGMA method revealed that clustering of populations was strictly consistent with the geographic distribution. In conclusion, the genetic diversity of C. sphinx was high, population history was long, most populations were heterozygote deficiency and significantly departure from Hardy-Weinberg equilibrium. Maybe these were correlated with habitat fragmentation by human activities.