Isolation, Identification And Anticancer Actives Of Tribulus Terrestris L.

The plant Tribulus terrestris L.(Zygophyliaceae) is used in folk medicine.The steroidal saponins is active part of T.terrestris Lin..The application of reactive component like saponin in T.terrestris included the pharmacdogical action on cardiac and cerebral vessels,inhibition senility and sexual behavior increasing.The preparation for increasing sexual behavior and softening blood vessel had been researched in the Overseas.In our country market had already appeared the preparation of Xinnaoshutong which active part was steroidal saponins extracted form total grass of T.terrestris.It is necessary to research the chemical compounds to form the active compound.To established the method of content determination of crude drugs of T. terrestris.Report related to monomer active component of Tribulus terrestris L.utilized in anti-oncoma was extremely little.Based on chemical composition of the entire plants of T.terrestris L.and advancement of pharmacological research,Compoundâ… ,â…¡,â…¢,â…£were isolated from it by using open column (SiO_z,Sephadex LH-20),preparative HSCCC methods et al..And compoundâ…¢was racemic compound.Furthermore,Compoundâ…£was isolated from racemic compound with the technology of chromatographic technique HSCCC.And according to spectroscopic analysis, including IR,¹H-NMR,¹³C-NMR,ESI/MS,H-¹H COSY,HMBC,NOESY,HSQC and chemical methods,the structure of Compounds were identified as:(â… )β-sitosterol,(â…¡)13-sito sterol-β-D-glucopyranosyl,(â…¢)(R,S)Hecogen in-3-O-β-D-glucopyranosyl(1-2)-[β-D-xylopy ranosyl(1-3)]-β-D-glucopyranosyl(1-4)-β-D-galactopyranoside,(â…£) Hecogen in-3-O-β-D-gluco pyranosyl(1-2)-[β-D-xylopyranosyl(1-3)]-β-D-glucopyranosyl(1-4)-β-D-galactopyranosideAccording to pharmaco-experiment of Compoundâ… ,â…¡,â…¢andâ…£,the result showed: only Compoundâ…£restrained selectively the growth of Wish cell.Furthermore,it poisoned dramatically to MCF7,Be17402,BGC823 and Caco-2.There was not evident activity for Compoundâ…£and its compound constructed with itself' isomeride.The reasons of phenomenon was analyzed,however,if its relationship between chemical constitution and pharmacological activity was revealed,the further research would be engaged.The science theory was provided for the puncturevine pharmacodynamics function and the action mechanism research of T.terrestris and the puncturevine innovation medicine development.