The Fabrication Of Cell Culture Chip And Pilot Study Of Mesenchymal Stem Cells Differentiation On-chip

Miniaturized Total Analysis Systems(μTAS) is becoming more and more important as an interdisciplinary technique.Due to its advantages,microfluidic chip has been applied in cytological researches in recent years.In this dissertation,the fabrication technique of different kinds of microchips has been studied,including chip design,glass etching,glass bonding,temperature control and sample injection.We tried materials of both glass and Polydimethylsiloxane (PDMS)for glass-glass and glass-PDMS microchip fabrication.These chips can successfully retain cells for cell cultures.In our experiment,mesenchymal stem cells (MSCs) are used for the test of on-chip stem cell differentiation.Detailed results of this research are concluded as below.1.Microchips of glass or glass-PDMS or PDMS were successfully fabricated using standard photolithography,wet etching and high-temperature bonding techniques.The temperature was precisely controlled for on-chip cell cultures.2.A large-scale logarithmic flow-rate damping microchip was proved efficient for cell retaining of different adherence.3.MSCs were cultured for osteogenic and adipogenic differentiation details. These cell differentiation details provided useful background or comparable data for further on-chip cell differentiation studies.4.On-chips MSCs culture was achieved as a preliminary study.Cells on chips were able to proliferate.This on-chip cell culture technique provide a useful platform for on-chip cell studies.Our research idicated that cells can relive on chip,and it can be observed by microscope camera system simultaneity.However,some experiment conditions must to be research if cells culture on chip long time.