| The actin-depolymerization factor /cofilin family is a class of actin-binding protein which exists in eukaryotic cell, they cut off the microfilaments by increasing the depolymerization of actin filaments, increase the concentration of G-actin to rearrangement the cytoskeleton, in turn they affect many important physiological functions such as cell migration, cell proliferation and apoptosis. In mammal, actin-depolymerizing factor/cofilin family include actin depolymerizing factor (destrin/Dstn /ADF), non- muscle cofilin (cofilin1) and muscle cofilin (cofilin2). Follicular development is an important case in reproduction process, follicular dysplasia will lead to ovulation abnormally or failure, eventually bring about infertility. Until now, there is no article about the expression and regulation of the actin depolymerization factor/cofilin during follicular development in mammal.The present study use in situ hybridization, realtime PCR and immunofluorescence to investigated the expression and regulation of ADF, cofilin1 and cofilin2 in mouse follicular development with modle of sexual maturation, modle of follicular development induced by hormone. During follicular development induced by PMSG, cofilin1 mRNA express in granulosa cells of secondary follicle which taking place proliferation after 0,6,12,24 hours of PMSG injection, these results prompt cofilin1 may be related to the proliferation of granulosa cells. During follicular development induced by PMSG/HCG, cofilin1 mRNA express in granulose cells of mature follicle and corpus luteum after 12,18,24hours of HCG injection.The results of realtime PCR at the same time period indicate that expression of cofilin1 mRNA is higher in granulose cells of ovulation phase, these results prompt cofilin1 may be involved in granulosa cell differentiation,and contribute to ovulation and luteinizing. The expression of cofilin1 mRNA is similar with hormone treatment modle in mouse sexual maturation ovary.The results of in situ hybridization show that cofilin1 mRNA express in oocyte.The results of immunofluorescence further confirm cofilin protein get close with the nuclear chromosome . These results prompt cofilin may be related to chromosome function.During follicular development induced by PMSG, Dstn mRNA express in follicular theca of secondary follicle and tertiary follicle after 0,6,12,24,48 hours of PMSG injection, these results prompt Dstn may be related to function of follicular theca. During follicular development induced by PMSG/HCG, Dstn mRNA express in follicular theca after 0,6,12,18 hours of HSG injection. Corpus luteum has been formed in ovary after 24,48hours of HCG injection. Dstn mRNA Express in corpus luteum. The results of realtime PCR at the same time period indicate that expression of Dstn mRNA is higher in luteinizing granulosa cells. These results prompt Dstn may participate in the formation of corpus luteum. The expression of Dstn is similar with hormone treatment modle in mouse sexual maturation ovary.Each stage of follicular development, cofilin2 mRNA express very weakly.Our results show that cofilin1 and Dstn may play an important role in mouse follicular development.
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