| Objective: Study on Chinese medicine Ginkgolide B induces the differentiation of embryonic stem cell into cardiomyocytes in vitro. Provide experimental evidence for transplantation of stem cell in the treatment of myocardial illness. Aimed at providing theoretical basis for the development of biological resources of stem cell and the new role of traditional Chinese medicine.Methods: Using the classic"three-steps way"to culture embryonic stem cells, add three different final concentrations of Ginkgolide B(10-5mol/L,10-4mol/L,10-3mol/L)into the differentiation culture at the same time. The morphology of cells was observed under invert microscope; specificα-sarcomeric actin was detected with immunocytochemi- stry; differentiation ratio of ESCs was detected by flow cytometry .Results :(1) It can be observed that the embryonic bodies begin automatic rhythm of contraction from the eighth day , reach a peak on the thirteenth or fourteenth day. The beating frequency between Ginkgolide B groups and the control group showed no significant difference (p > 0.05). (2)Cardiac specificα-sarcomeric actin was positive. (3) The concentration of Ginkgolide B-induced embryonic stem cell differentiation is 10-4mol/L, and they have significant differences with the control group(p < 0.05).Conclusion: (1) Ginkgolide B has obviously effect on the differentiation of embryonic stem cells into cardiomyocytes . (2) Ginkgolide B has no effect on the beating frequency of the differentiated cardiomyocytes.
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