Polyphasic Analysis Of Two Anaerobic Bacteria

Five anaerobic bacteria were isolated from the four samples as follows: sediments of a soda lake in Jilin province,waste water of hot pickled mustard tuber, sediments of Aqike lake in Xinjiang autonomous region and sediments of rich-cobalt region of West Pacific.Based on the 16S rRNA gene analysis,they belong to the four genus of Amphibacillus,Virgibacillus,Aerococcus and Desemzia.Strain Y1^T was isolated from sediments of a soda lake in Jilin province and its 16S rRNA gene number in GenBank was FJ169626.Comparative 16S rRNA gene analysis on Eztraon website revealed 93.4-96.8%sequence similarity to members of the genus Amphibacillus.Strain Y1^T was facultatively anaerobic,terminal spore-forming and Gram-positive staining rods;not dependent on Na⁺ but highly halotolerant;the optimum pH for growth was 9.0,with a range of 7.5-10.5;mesophilic with optimum growth at 32℃;be capable of utilizing certain mono- and oligosaccharides and hydrolysing soluble starch and casein;MR-VP tests,catalase and oxidase activities were negative;the predominant fatty acids were branched anteriso-C_15:0 and iso-C_15:0. It was a member of clusterâ…ªwithin the low DNA G+C Gram-positive bacterium and the DNA G+C content was 37.7 mol%.Based on the polyphasic analysis,strain Y1^T represents a novel species of the genus Amphibacillus,for which the name Amphibacillus jilinensis sp.nov.is proposed.The type strain is Y1^T(=CGMCC 1.5123^T=JCM 16149^T).The other strictly anaerobic and halophilic bacterium isolated from sediments of Xiaokule salt lake in Xinjiang by Kong-Lan Shi,named E2R^T was also discussed in this paper.The 16S rRNA gene number of the isolate in GeneBank was FJ169626. Similarity analysis based on the 16S rRNA gene sequences revealed that strain E2R^T was most closely related to members of the genus Alkaliphilus with 95.5-91.1% sequence similarity.Further research indicate that strain E2R^T represents a novel species of the genus,for which the name Alkaliphilus halophilus sp.nov.is proposed. The type strain is E2R^T(=CGMCC 1.5124^T=JCM 16124^T).