Raman Study On Biomolecular And Bacteria Research

The atomistic specificity of vibrational surface-enhanced Raman Spectral signatures provides a powerful and effective method for molecular framework information. In this article, the interation of DNA and norfloxacin, DNA and shikonin have been researched, as well as glycine calcium complex, Escherichia coli BL21 and the recombinant E. coil BL21 (pET32-nrdH). The main contents are as follows:(1) FT-Raman and surface enhanced Raman Spectra (SERS) of norfloxacin and norfloxacin capsule on the silver colloidal substrate are recorded, the vibratonal and enhanced peaks are assigned; interactions of norfloxacin and DNA are researched. The experimental results proved that characteristic vibrations of capsule's content and NFX standard, which is v(C-F), vs (O-C-O), v(C=C), haven't changed, while the changes are molecular skeleton's vibrations. Addives doesn't affect the raman spectroscopy of norfloxacin substantively, a analytical method by which norfloxacin medcine is detected can be established by FT-Raman. Without any irons, by which the inserting-action mode is the ring of norfloxacin insert the double helix structure of DNA, norfloxacin could interact with DNA derectly.(2) The paper analyzes the ingredient of Shikonin in Lithospermum by associating hyphenated high-efficiency thin layer chromatography(TLC) with surface-enhanced Raman spectroscopy(SERS) techniques, the SERS of shikonin-DNA and interaction of separate shikonin TLC SiGF254 with DNA is researched. The results show that characteristic spectral bands of shikonin can be obtained from the TLC spot with SERS by silver nanoparticles, and also the interacting way of shikonin with DNA:ring of shikonin insert the double helix structure of DNA.(3) In the paper, the Fourier transform Raman spectroscopy (FT-Raman) of Glycine is researched. And Glycine calcium complex was studied by IR and Raman spectroscopy.(4) Surface enhance-Raman Spectra were acquired from Escherichia coli BL21 in its growth cycle (including lag phase, logarithmic phase and stationary phase) and SERS of the recombinant E. coil BL21 (pET32-nrdH) in stationary phase is obtained by using Fourier transform infrared (FT-IR) Raman spectroscopy, the vibratonal and enhanced peaks are assigned. The results showed that SERS of them due to DNA and protein molecular vibrations of cells and they are different, and can be identified.