The Biotransformation Of Glucoraphanin And Glucoraphenin And Study Of Stability Of Sulforaphane Degradate From Glucoraphanin

Glucosinolates are an important class of secondary compounds found in the seeds, roots, stems, and leaves of plants belonging to dicotyledonous angiosperms. They have much effectiveness as a pesticide, anti-microbial reagent and natural fumigated disinfector. As one kind of degradation products, Isothiocyanates have shown a series of biological and chemical characteristics. Besides their special flavor for condiments, they have many biological properties such as anti-bacterial, anti-carcinogenic potential. Isothiocyanates can protect DNA-damage and cancer caused by many dietary carcinogens such as polycyclic aromatic hydrocarbons, heterocyclic amines and nitrosamines. These properties have attracted the interesting of many food chemists, medicinal scientists and biologists. In the present dissertation, glucosinolates and their degradation products in traditional Chinese medicine were investigated and the purification and hydrogenation of glucoraphenin extracted from the seeds of Raphanus sativus L. were studied.Firstly,32 bacteria were evaluated for the capacity of biotranformation of glucoraphanin and glucoraphenin. The results showed that high capacity of biotransformation of glucoraphanin was found in 25 bacteria and the highest capacity was found in Alternaria alternate and Cylindrocarponsp. In addition, the data showed that high capacity of biotransformation of glucoraphenin was found in 4 bacteria and the highest capacity was found in Cephalosporium core mioides.Secondly, the biotransformation condition of biodegradotion of glucoraphanin has been studied, and the optimum cultrure condition is 20mg/ L substrate, in 25℃,160 rpm,96 hours, glucose 1% and pH6.5-7.5 in each suspension 60mLThirdly, through single factor experiments on the biotranformation of may affect the glucoraphenin by Cephalosporium core mioides, the optimum cultrure condition is 96 hours after administration, pH6-8, dosage was 40μg/ml. Transformation products were identified by HPLC,UV and LC-MS the results shows that the biotransformation product was glucoraphanin.Finally, through a serious of experiments, the stability of sulforaphane was observed. The concentrations of sulforaphane was determined by HPLC. The stability of sulforaphane and its solution under different temperature, light, air, oxygen and different packaging packaging materials was studied. Experiments show that the longer heated to high temperatures occur sulforaphane content significantly decreased, degradation products were increased. The thermal instability of sulforaphane, with increasing temperature or heating time will gradually decompose. On the light stability. Oxygen in the air can accelerate the degradation of sulforaphane, packaging materials on the stability of sulforaphane little effect.