SPC-neu-polyA Transgenic Mice Establishment And Phenotype Identification

Objective:Lung cancer has currently become the No. 2 killer in cancers threatening human health. Our study was to establish a transgenic mice model integrated with an exogenous SPC-neu-polyA construct, identify their phenotype, so to investigate how the neu gene work in lung cancer development and the relationship between pneumonia and lung cancer.Methods:Our study included two parts. First, The SPC-neu-polyA sequence was microinjected into mice to generate transgenic mice, which were identified by PCR, Southern blot, Western blot, FISH and immunohistochemistry. Second, the phenotype of transgenic mice was identified by histological HE staining, Real Time-PCR and IHC. Supplement expreiment:10 cases of lung cancer biopsy specimens were detected by IHC to analyze the neu protein expression and inflammation factor expression.Results:The results of PCR, Southern blot and FISH suggested that SPC-neu-polyA was completely integrated into the genome of transgenic mice, and located in the 16th Chromosome. The results of Western blot suggested that the neu protein was expressed in transgenic mice; IHC experiments suggested that neu protein was expressed in the transgenic mice's alveolar and bronchial epithelium. Significant inflammatory changes were found in the transgenic mice but not in the normal mice by Pathology HE staining. Some transgenic mice showed epithelial cell hyperplasia, one of precancerous lesions. Immunohistochemistry detection of 10 cases of lung biopsy specimens of lung cancer patients showed neu-positive and the expression of inflammatory factors.Conclusions:From these results, SPC-neu-polyA transgenic mice had been successfully established. Their phenotypes included inflammatory changes and precancerous lesions, indicating they were inflammatory disease models. The animal model can be used to study the role of neu in lung cancer development and the relationship between pneumonia and lung cancer.