Effect Of NaCl Stress On Electrical Impedance Spectroscopy Parameters And Chlorophyll Fluorescence Characteristics Of Moso Bamboo (Phyllostachys Edulis) Seedling Leaves

Moso bamboo (Phyllostachys edulis), a perennial plant, belonging to Phyllostachys in Gramineae Bambusoideae, is one of the important economic species of bamboo. They also have high economic benefit, as well as social benefit and ecological benefit. In this paper, the effects of NaCl stress on parameters of electrical impedance spectroscopy (EIS), the physiological indicators and the chlorophyll fluorescence characteristics of Moso bamboo seedling leaves were investigated. The aim of the study was to verify the relation between the parameters of EIS and salt tolerance, and between parameters of EIS and those of physiological indicators, and analyse the changes of chlorophyll fluorescence parameters of Moso bamboo seedling leaves under NaCl stress and understand the effects of NaCl stress on the photosynthetic mechanism of Moso bamboo seedling leaves.The main results are as follows:1. With increase of NaCl concentration and long time stress, change of electrical impedance spectroscopy and EIS parameters of Moso bamboo seedling leaves occurred.2. There were significant negative correlations between extracellular resistance, relaxation time and change of membrane permeability of Moso bamboo seedling leaves under NaCl stress (The coefficient of determination was R~2=0.75 and R~2=0.76, respectively).3. There were significant negative correlations among EIS parameters extracellular resistance, intracellular resistance, relaxation time and physiological indicators proline content as well as SOD activity of Moso bamboo seedling leaves under NaCl stress (The coefficient of determination was R~2=0.66, R~2=0.95, R~2=0.88 and R~2=0.69, R~2=0.70 and R~2=0.80, respectively).4. There were significant positive correlations among extracellular resistance as well as relaxation time and POD activity of Moso bamboo seedling leaves, while the negative correlation between distribution coefficient of relaxation time and POD activity of Moso bamboo seedling leaves was found under NaCl stress (The coefficient of determination was R~2=0.70, R~2=0.76 and R~2=0.51, respectively).5. There were significant positive correlations among extracellular resistance, intracellular resistance, relaxation time and MDA content of Moso bamboo seedling leaves under NaCl stress (The coefficient of determination was R~2=0. 89, R~2=0.55 and R~2=0.59, respectively).6. There were significant positive correlations between distribution coefficient of relaxation time and content of soluble protein under NaCl stress.7. With increase of NaCl concentration and long time stress, the chlorophyll fluorescence PARameterΦPSⅡ, ETR, Fv/Fm, and qP decreased, whereas qN increased firstly, and then decreased.8. High NaCl concentration (≧0.35%) stress inhibited photosynthesis and starting rate of photochemical reaction, and reduced light utilization efficiency of Moso bamboo seedling leaves.