Research On Identification And The Antimicrobial Active Of A Marine Anaerobic Sulfate-reducing Bacterium S130 (3)-2

With the advance of medicine, and developing of new drugs, terrestrial microorganisms, the traditional resources of antibiotics, enzyme inhibitors and other bioactive substances, seemed to be almost exhausted. The rate of discovering of new drugs significantly slowed down and the rate of technological input-output declined. New drug source were in great demand. All species in the ocean have been found, including bacteria, actinomycete and marine fungi. A wide variety of marine microorganisms and their specific bioactive substances determined the potential is enormous, which would be as the bacteria producing biological active substance. For their special living environment, marine microorganisms have unique genetic background and metabolic pathways, and were found to be potential to synthesize some structurally distinct antibiotics. Therefore they have become a significant source for new antibiotics of screening.An antimicrobial marine sulfate-reducing bacteria S130(3)-2, isolated from the sea water sample of Heishijiao sea area in Dalian, was studied in this paper. Different fermentation conditions and antibacterial activity were investigated and a method was set up by using macroporous resin to extract the antimicrobial active substance. Main results are as follows:By studying culture conditions of strains, the best culture conditions were established: initial pH was 7.2, the temperature was between 25℃and 30℃, the optimal culture time was 28 d.The suitable extraction scheme of macroporous adsorptive resin was as the following: first fermentation broth was treated by sand filter, subsequently adjusted pH 6, extracted with AB-8 resin column. The optimum fermentation broth and resin weight ratio was 20 / 1, with 95% ethanol eluting 4 column volumes.By comprehensive using column chromatographies on silica gel, Sephadex LH-20 and reverse phase silica gel and high performance liquid chromatography, three pure compounds and a number of active component were obtained. The compounds WS-4-1-1-4-2-3 and WS-4-1-1-7-3 were indentified as diisobutyl phthalate and butyl phthalate by spectral analysis of 1H-NMR, 13C-NMR as well as by comparison with data reported in reference. For its bad purity, less useful information provided by MS and 1H spectrum, the compound WS-3-16-1 was failed to be identified.