The Research Of Bacteria Diversity In The Rocky Desertification Area In Guizhou Province

The soil samples were collected from rocky desertification areas, Anshun,Bijie in GuiZhou province and the southwest of GuiZhou .The extraction effects of the desertification environment soil DNA were designed and compared among TENS-ammonium acetate direct method, SDS high-salt direct method, indirect method and kit extraction method .The template of follow-up experiments was soil DNA extracted by kit extraction method .The follow-up experiments as follows: metagenomic DNA of soil samples extracted from 6 soil samples was amplified by 16SrDNA; PCR products were purified from agarose gel and linked into pMD-18T vector and transformed into host strain DH5α; positive clones were selected through blue and white blots test; plasmids were extracted; 23 positive clones plasmids were selected randomly and sequenced by biology company ,the sequenced results compared with the similar sequences searched by BLAST tool in the GeneBank on NCBI to get the highest similar bacteria .Finally, phylogenetic tree was constructed by using ClusterW2 on-line multiple sequence alignment. The similarity comparison results and phylogenetic analysis reveal that microorganisms are limited in the special desertification environment of Guizhou. They are nearly Citrobacter except three bacterias not to be found in the GeneBank .So the dominant bacteria is Citrobacter.