Interleukin-6 Inhibits The Expression Of Apolipoprotein M In HepG2 Cells

Objective:Apolipoprotein M (apoM) is a recently discovered human apolipoprotein predominantly present in high-density lipoprotein (HDL) in plasma, exclusively expressed in liver and in kidney. The human apoM gene is located in the major histocompatibility complex classâ…¢region on chromosome 6. Recent studies have shown that apoM has an antagonism effect on atherosclerosis. So it may be beneficial on the Macro-vascular Complications in Type 2 Diabetes Mellitus.And the serum levels of Interleukin-6(IL-6) are significantly increased in type 2 diabetes with coronary disease in recent many studies. So we investigated the effect of Interleukin-6 (IL-6) on apoM expression in a human hepatoblastoma cell line (HepG2) in the study and reseach the pathogenesis of the the Macro-vascular Complications in Type 2 Diabetes Mellitus.Methods:1.In this study,.HepG2 cells were cultured and incubated with different concentrations of IL-6 (0,1.25,2.5,5,10,20,40,80ng/ml) for 24 hours. After the incubations, total RNAs were extracted and applied to reverse transcript PCR, real-time quantitative PCR and Western Blotting to detect the expression levels of apoM and apoA-I.2.HepG2 cells were incubated with 2.5ng/ml IL-6 in different time(0h,1h,3h,6h,12h,24h).After the incubations, total RNAs were extracted and applied to reverse transcript PCR and real-time quantitative PCR to detect the expression levels of apoM.The effect of IL-1a on apoM expression was also determined.Results:1.IL-6 inhibited the expression of apoM significantly (F=10.778,p<0.01). Whereas IL-6 did not influence the expression of apoA-I (F=2.004, p>0.05).IL-1a (0,1.25,2.5,5,10,20,40,80ng/ml) did not decrease the expression of apoM (F=2.038,p>0.05).2.IL-6 can inhibit the expression of apoM in 12 hours and in 24 hours.(n=8, F=116.805,p<0.05).And the inhibiting effect is not clear in shorter time than 12 hours.Conclusion:IL-6 can inhibit apoM mRNA transcription in the HepG2 cell.And the effect is characterized by particularly and saturability. It may contribute to the pathogenesis of abnormal lipid metabolism and macrovascular complications in type 2 diabetes. The pathogenesis of the the Macro-vascular Complications in Type 2 Diabetes Mellitus is very conpliticated. It is caused by many facts. We investigated the effect of Interleukin-6 (IL-6) on apoM expression in a human hepatoblastoma cell line (HepG2) in the study and associated the inflammation facts and abnormal lipid metabolism to lay a new foundation for further study.