A Way Of Making Substrate Material And Bases Test Of Surface Enhanced Raman Spectroscopy

A spectroscopic assay based on surface enhanced Raman scattering (SERS) using silver nanorod array substrates has been developed that allows for rapid detection of trace levels of viruses with a high degree of sensitivity and specificity. This novel SERS assay can detect spectral differences between viruses, viral strains, and viruses with gene deletions in biological media. The method provides rapid diagnostics for detection and characterization of viruses generating reproducible spectra without viral manipulation, but the key of this spectroscopic assay is how to make the practical,feasible and effective substrate material for SERS.This paper chooses the tiny balls of polystyrene as the substrate material of SERS. The size of the tiny balls of polystyrene ranged from 150 to 250 nm (mean 200nm) in diameter, through chemical and physical methods it deposits a layer of Ag on the tiny balls, and then use it to to detect and measure Raman spectrum of pyrimidine and purine bases.