Study On Enhancement Of Long-PCR Specificity By Nanoparticles

Nanobiotechnology, based on life sciences and material sciences, is a hot and foreland topic in international biotechnology area.The scientist come from all of the world study the new technology from different perspectives.In 2005, scientists of Shanghai Jiao Tong University demonstrated that the gold nanoparticles increase specificity of PCR (Polymerase Chain Reaction) significantly. Shortly, a research come from Taiwan, China found that the gold nanoparticles could enhance specificity and sensitivity of PCR significantly. Except for a successful application of nanotechnology in biotechnology, the more important is that this achievement presents a new visual angle in research about how to find more new and highly efficient PCR additives.So, based on the previous research, this thesis explored the effect of a series of nanomaterials on PCR, and the potential mechanisms were also studied and discussed.In this thesis, a Long-PCR model was set up, and 16 kinds of nonmetallic nanonmaterials were tested. The results demonstrated that there are 12 kinds of nanomaterials that could directly increase the specificity of PCR, and they are different kinds of carbon nanopowders.This thesis also employed the mixed PCR addictives to improve the low products induced by nanoparticles. The results demonstrated that the compound of trehalose and nanoparticles is not only beneficial for specificity but also beneficial for PCR yields.There are dozens of small chemicals that participate the yeast cellular metabolic, and code them with number. Four of them could enhance the general PCR. Every of the small chemicals mix with 16 nanoparticles to join in the Long-PCR system, the mix addictives could increase specificity of PCR. At the same time, they could increase the production of PCR. Meanwhile, the mix additives which contain No.23 (Inosine) were the most efficiency.The mechanism of action that nanoparticles could enhance PCR is that the enhancement of heat equilibrium caused by nanoparticles and interaction of nanopartiles with reaction components were likely to be responsible for PCR enhancement.