Characterization Of The Hydrolysate Of Agriculture Waste By Xylanase And Diluted Acid For L-arabinose Production

This thesis describes the hydrolysis of agriculture wastes such as bagasse, sugar beet pulp, corn cob, and cassava dregs with a thermostable xylanase and dilute sulphuric acid, for the rare sugar production. HPLC has been adopted for the analysis of hydrolysates. The results show that L-arabinose, glucose, and xylose were obtained from sugar beet pulp, corn cob, and cassava dregs. However, only glucose and xylose were harvested from bagasse hydrolysis. The highest L-arabinose productivity was obtained from the hydrolysate of sugar beet pulp. Using sugar beet pulp as the substrate, the hydrolysis condition was optimized for L-arabinose production. In the enzymatic reaction of 60℃for 48 hours with 200 IU/mL xylanase, then followed by 2 % dilute acid hydrolysis at 100℃for 1 hours, the highest productivity of L-arabinose was up to 50.8 % in total monosaccharides. This thesis has described the xylanase production by Thermomyces lanuginosus DSM10635 under corn cob solid substrate fermentation, and a few works of screening microorganisms which were absented the L-arabinose metabolism pathway also.