Extraction, Separation And Purification Of The Active Compositions From Spirulina Platensis(Ⅲ)

The Spirulina Platensis is the new plant protein resources, which has aroused general concern since 1970s. The algae open up broad prospects for the food additives, health protection foods and pharmaceutical raw materials. Although there are many investigations on research and development of Spirulina Piatensis in China, especially its cultivated technology having caught up with and surpassed the advanced word levels, its further products and exploiting research lag behind and industrial production is still to have further studies. Scientific investigators have extracted, separated and purified the attractive C-phvcocvanin (C-PC),polvunsaturated fatty acied v -linolenic acid (GLA), P -carotene from spirulina platensis. while there is less research on other aspects. In order to make it use extensively, we have dwelt upon the synthesis and purification of phycocvanobilin dimethvl ester coming into use in the fluorescent quarantine.Phycocvanobilin dimethvl ester, a kind of bright-colored blue pigment, is synthesized by esterification reaction using phycocvanobilin and methanol. It has the same quality with phycobiliprotein. It can be used to manufacture fluorescent probe and widely used in clinical diagnoses, e. g. cancer ,leukemia and the research of genetic engineering. The synthetic ways and means of phycocvanobilin dimethvl ester are: first, extracting phycoeyanobilin from spirulina platensis by using of surface active agent, then dissociating sulfuric ether bonds with methanol and obtaining phvcocvanobilin , at last, with phvcocvanobilin and methanol, under the reaction of catalyst gaining phycocvanobilin dimethyl ester. In the course of the experiment, we selected three kinks of catalyst with excellent performance andIIexamined it adopting orthogonal experiments. While the POCl~ used as catalyst, the optimum conditions are: reaction temperature is 550, reaction time is thirty minutes, proportion of phycocyanobilin to methanol is one to one hundered, proportion of catalyst to methanol is six to one hundred, under these conditions, the yields is 42. 8%. It is very con\'enient to use POCl:~ as catalyst, but its yield is lower than that of using other two catalysts. As the SOCl2C~1-kN used as catalyst ,the optimum conditions are :reaction temperature is 50C, reaction time is thirty minutes, proportion of catalyst to methanol is six to one hundred, proportion of ph~coeyanobilin to methanol is one to one hundred and the yields is 53. 7%. Though the yields is higher by using of this way, this kind of catalyst is not prepared easily. Furthermore, it is much more trouble to deal with later stage of reaction. When the HA1CD2Et2O adopted as catalyst, the optimum conditions are: reaction temperature is 40C, reaction time is fifteen minutes, proportion of catalyst to methanol is fourteen to one hundred (v v), Proportion of phvcocvanobilin to methanol is one to one hundred and the yields is 47. 7%, under these conditions, its reaction temperature is lower than that of using other two kinks of catalyst and its reaction speed is faster than that of them and the catalyst is easy to be prepared. So it is suitable for esterification reactions in the laboratory and a certain scale manufacture.Phycocyanobilin dimethyl ester is purified by thin layer chromatography or column chromatography on silica gel using the solvent system carbon tetrachloride-ethvl acetate (2:l,v;v). Its UV, IR, Ms spectral data are compatible with those in the literature, Its conformation variation in solutions is studied.