Strong Anion-Exchange Capillary Electrochromatography And Capillary-Based Metal Chelate Enzyme Reactor

Capillary Eletrochromatography(CEC) is a high efficiency nucro-separation technique which combines the selectivity separation of solutes in HPLC with the high efficiency of electro-osmotic driven separation methods such as CE. In this study, the separation of acidic compounds by strong anion-exchange capillary electrochromatography (SAX-eEC) was carried cut. The effect of the absorption of the analytes onto the stationary phase, applied voltage and ionic strength of the buffer on the retention behavior was investigated. The difference of the separation mechanism between SAX-CEC and CE was discussed. We constructed a fused-silica-capillary-based metal chelate micro-reactor with immobilization of trypsin. To combine the micro-reactor and matrix-assisted laser desorptionlionizaiicn time of flight mass spectrometry (MALDI-TOF-MS), peptide mapping for nano-liter protein sample can be performed. The advantages of the prepared micro-reactor are easily to be stored and regenerated.