| Resonance Rayleigh scattering (RRS) is a new technology developed in recent years. For its remarkable characteristics of high sensitivity, high selectivity and simple operation, this method brings to more attention and interesting and has been applied widely to the determination of biological macromolecules such as nucleic acids and proteins. Moreover this technology has been used to the study and determination of trace amounts of inorganic ions. However, there is rarely report about this technology used to the analysis of antibiotic drugs. In this paper, the RRS spectra and their application of the new five systems such as chlorpromazine hydrochloridum-nucleic acid systems, aminoglycoside antibiotics-nucleic acid systems, cation surfactants-nucleic acid systems, some bisazo dye-aminoglycoside antibiotic systems and La3~-tetracycline antibiotics systems for the determination of nucleic acids and antibiotics have been studied. The spectral characteristics, the influencing factors, the properties of analytical chemistry and their analytical applications have been investigated. The new, simple, fast and accurate methods for the determination of nucleic acids and antibiotics have been developed. Among them, the cation surfactant- nucleic acid systems can be applied to the determination of nucleic acids at nanogram levels. Gentamicin and kanamycin in the aminoglycoside antibiotics-nucleic acid systems have very high specificity for the determination of DNA and have no signal of RRS for RNA. La>-tetracycline antibiotics systems have very high selectivity for the determination of chlortetracycline (CTC) and doxycycline (DOTC). Other tetracycline antibiotic drugs with similar structures and properties such as oxytetracycline (OTC) and tetracycline (TC)haven't such RRS response to La~ The experimental results are consistent with the results calculated by semi-empirical program of quantum chemistry, AM1 method. 1. Chlorpromazine hydrochloridum- nucleic acid systems In Britton-Robinson buffer solution of pH 2-3, the reactions of nucleic acids with chlorpromazine hydrochloridum can produce intensive signals of RRS, which its maximum RRS peak is located at 400 nm. Taking ctDNA for an example, the suitable reaction conditions and the selectivity of the method have been studied. The intensity of RRS is directly proportional to the concentration in the range of 0-4.0 j.tg/mL for DNA and 0-8.2 tg/mL for RNA. The method has high sensitivity, and the detection limit is 7.6 ng/mL for ctDNA, 11.3 ng/mL for hDNA, 11.5 nglmL for sDNA and 28.4 ng/mL for yRNA. The method has been applied to the determination of nucleic acids in four synthetic samples with high accuracy and good repetition. 2. Aminoglycoside antibiotics- nucleic acid systems In weak acidic Britton-Robinson buffer solution, the reactions of nucleic acids with some aminoglycoside antibiotics such as kanamycin (KANA), gentamicin (GEN), tobraniycin (TOB) and neomycin (NEO) can produce intensive signals of RRS. The Resonance Rayleigh Scattering spectra of the four systems are similar and their maximum RRS peaks are all located near 470 nm. The linear ranges for the determination of nucleic acids are wide and the intensity of RRS is directly proportional to the concentration in the range of O0.0 tg/mL for ctDNA, 0?6.0 pg/mL for yRNA. The method has high sensitivity and the detection limit tctDNA is 13.2 ng/mL (GEN), 17.4 ng/mL (KANA), 11.4 ng/mL (TOB) and I 3.8 ng/mL (NEO). The detection limit for hDNA is 15.6 ng/mL (GEN). 20.6 ng/... |
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