Application Of Flow Injection Analysis And Flow Injection Analysis Combine With Chemometrics In The Dertermination Of Pharamaceuticals

This thesis studies the analytical methods of determination for Sucralfate, Calcium Dobesilate , Piracetam , Diphenhydramine hydrochloride , Sulfamethoxazolum and Fosfomycin by Flow injection .Some Chemometrics methods combine with Flow injection analysis are utilized for the optimization of variables such as Simplex and Factor design.The methods have been applied to determine the samples and satisfactory results were obtained.lt includes fiveparts as follows:Part I : Review. A review of the application of Chemometrics in Flow injection analysis given.Part II: Determination of Sucralfate usingflow injection inhibitochemilumin-escence. A novel flow injection inhibitory chemiluminescence system for the determinat of Sucralfate was presented. While a volume of Calcium Dobesilate mixed with a volum of NaCIO, by the fast oxidation reaction between Sucralfate and NaCIO, NaCIO was generated,which then inhibited the CL reaction of NaCIO and luminol in alkaline queous solution. The CL emission was correlated with the Sucralfate concentration in the rangeS.OX lO'M.OX 10~5 g-mL"1; The detection limit was (3o) 1.6X10~7g-mL"'; The relative standard deviation was 1.6% (Sucralfate 1.0X 10"6 g-mL'1, n=l 1); A sampling frequency of 200 h''was attained. 0Part Hit Determination of Calcium Dobesilate using flow injection inhibito-chemiluminescence. A novel flow injection inhibitory chemiluminescence system for the determinat of Calcium Dobesilate was presented. While a volumeof Calcium Dobesilate mixed with a volum of K3Fe(CN)6,by the fast oxidationreaction between Calcium Dobesilate and K3Fe(CN)6, K3Fe(CN)6 was generated,which then inhibited the CL reaction of K3Fe(CN)6 and luminol in alkaline queous solution.The CL emission was correlated with the Calcium Dobesilate concentration in the range 1.6X10~5~2.0 X 10'7g-mL''; The detection limit was (3o) 4,4X10"8g-mL"'; The relative standard deviation was 1.5% (Calcium Dobesilate 2.0X10"7 g-mL"1, n=ll); A sampling frequency of 60 h"1 was attained, oPart IV?Flow injection Spectrophotometry for the determination of Piracetam. To establish a method using flow injection Spectrophotometry, for determination of the content of Piracetam in drug-tablet^ injection and sirup. Used a duble line flow injection manifold, water is used as a carrier at a flow rate of 2.68mL-min" '.The conditions were 498nm detection wavelength,sample size 250uL and coil length 40cm. A sampling frequency of 90h"' attained. The calibration curves were linear in the range of 16~200ug-mL"'. The lowest limit of detection is 1 .Tug-mL"1. The average recovery is 99.8%, RSD is 1.0 (%). The new method is rapid, accurate, sample,sensitive and reliable. It is suitable for the assay of Piracetam by the factories and other institutions.Part V : Indirect Determination of Diphenhydramini by a continuous precipitation and stopped flow injection in drug formulations. A simple and rapid on-line flow injection precipitation flame atomic absorption spectrometric method for the determination of Diphenhydramine hydrochloride in drug formulations was established. This method was based on continuous precipitation with Bi(III)-iodide in acid condition. The precipitate was formed by injecting the Bi(III)-iodide into a carrier containing the sample and was subsequently retained on a membrane filter. By a stopped-flow mode with a 15s stopped-flow period, Diphenhydramine hydrochloride can be determined in the range 2.5~40ng-mL~',with a relative standard less than 3.5% and a sampling frequency of 60 h"1.Part VI?Computer-Assisted optimization for flow injection analysis of sulfamethoxazolum. A flow injection spectrophotometric method for the determination of the sulfamethoxazolum in drug formulators in the range l~10ppm was developed.p-Dimethylaminobenzalaminobenzaldehyde was employed to produce an yellow Schiff base absorbing at 450nm in concentrated hydrochloride acid medium. A factorial design program was utilized to establish the highly interacting factors that must be optimized. Optimizat...