Application And Study Of Resonance Light Scattering In Environmental And Biochemical Analysis

The author introduces a novel analytical method of the molecular emissionspectrometry field-the Resonance Light Scattering (RLS), and explores its applicationinto environmental and biochemical analysis fields in this thesis.We discuss various points of the existing theories, and study the present conditions which the terminologies of the RLS do not unify. We sum up the terms of the RLS in the documentary of recent years, and put forward the suggestion about this aspect.In the environmental analysis aspect, the author apply the RLS into the study on heavy metal elements of Mercury(II), Chromium (VI) deeply concerned by people in presentenvironmental pollution. Developing a new system of the RLS to determine Mercury-Hg( II) -SCN--RhB, the results of experiments show that Hg (II ) -SCN'-RhB system would appear the maximum intensity in the media H2SO4 and Amax 615nm, the linear range within 3.8 X 10-1~0.060ug/mL, determination limit is 0.38ug/L; The thesis also develops a new systemto determine Chromium(VI)-Cr (VI) -I'-RhB, this system would appear the maximumscattering intensity in the media HePOr and Xmax 610nm, the system has better linear within 3.1 X 10-3~0. 35u.g/mL, the determination limit is 3.1ug/L.The article also studies the optimum condition and the interfered situation when these two systems have other ions. The experiments lead to a conclusion that both systems are characterized by accuracy,simplify and a rapidity. We employ this method into the environmental analysis and compare with ripe method with satisfactory results.In the biochemical analysis aspect, it is first time to use the simple fluorescence spectrophotometer to determine the protein in human serum by red alizarin as the probe of the RLS. The experiment shows that the system HSA-red alizarin has the maximum intensity of the RLS in Britton-Robison buffer of pH4.10 at maximum wavelength of about 360nm. The method has better linear range within 0.040-10.0ug/mL, determination limit is 0.040ug/mL. Moreover, We have selected three other dyes of chromotrope 2R, acid chrome dark blue and pyrocatechin violet as the probes of the RLS in determining protein in human serum and achieving satisfactory results.