Biological-physical Hydrolyzing Means Of Pigskin Collagen

The production of hide collagen by protease has great economy value and important environment protection meaning. Fresh pigskin or residues of leather manufacturing was used as raw material. Three kinds of different technics were introduced, which were one-step technics, two-step technics (inhibition of protease activity) and two-step technics (not inhibition of protease activity). All kinds of influencing factors on dissolving ratio of pigskin, molecular weight distribution and viscidity of hydrolyzing product were studied, including dosage of 166 protease or AS 1.398 protease, reaction temperature and time, dissolving temperature and time, etc. It could be concluded that molecular weight distribution of hydrolyzing product of pigskin by protease was discontinuous, which differed from gelatin produced by alkali. The molecular weight of hydrolyzing product in two-step technics (inhibition of protease activity) exceeded 97KD mainly and its average value was larger than industrial gelatin. But smaller average molecular weight than industrial gelatin was attained in two-step technics (not inhibition of protease activity). The hydrolyzing technics affected molecular weight distribution of product more than other experimental conditions.At first, the activity inhibition of AS 1.398 protease and 166 protease remained in pigskin were studied, which was basic of two-step technics (inhibition of protease activity). The result showed that 166 protease had more stronger acid-resistant property than AS1.398 protease. And the inhibition of AS1.398 protease (or 166 protease) activity in pigskin could be achieved by disposal in the 0.2M Na2HPO4 -citric buffering solution (or 0.1N HC1) including 8% NaCl twice.At last, the means of removing other protein in the hydrolyzing collagen was primarily studied. We founded that other protein could be removed by ion exchange chromatograph, enhancing NaCl concentration of 0.05M Tris/HCl washing bufferingsolution which pH value is 7.5. And the stuffing of the chromatograph wasDEAE-cellulose.