Study On Enzyme-linked Immunosorbent Assay For Residue Of Chlorimuron-methyl

Chlorimuron-methyl of hapten had been synthesized ,then the haptenwere covalentlyconjugatedtobovineserumlbulmin(BSA)orhumanserumalbumin(HAS)or valbumin(OVA)to prepare antigens(immunogens)and coating antigens , The conjugates identified and their molecular ration of hapten to carrier protein were idetermined by UV spectrophoto metry ,Sodium dodecyl-sulfatepolycrylamide Gel Eletrophoresis(SDS-PAGE)and white rabbits were immunized with sythetic antigens the antisera are speafic to chlorimuron-methyl were obtained by using CM-BSAthe middileand final titers were l:12500and 1:62500 for anti-CM setum . the enzyme tagged IgG were prepared by coupling horseradish perxidase (HRP) o IgG.The enzymeconjugate maintained both immunological activity and enzyme activity.The half maximal inhibition to antibody antigen reaction were IC50 36.6ng/ml and limi of detection were IC2o0.7ng/ml for Chlorimuron-methyl. Cross-reactivities were determined for pyrazosulfaron metsulfuron-methylEthametsuIfuron-methyl chlorsulfuron was 0.1% , 3.2% 4.8% 0.27% Respectively,,Postgraduate:wangqinghua Major:Pesticide science Tutor:Prof.cheng tiebao...