| Cellulose is the most abundant and broad organic material in the earth. Not only the kind of precious resource is not made full use but also most of them are abandoned and then . became one of the pollution matter of the environment. In order to make full use of cellulose, experiments were carried out to study the screening of cellulolytic microbes and the production multi-microbe fermentation. 51 strains of cellulolytic microbes were isolated and screened from compost, cow faces and soil. 19 strains with high cellulase were gained by average growth velocity, filter paper decompostion and counter-action screening methodology, and then were used to co-fermentation experiments and identified.The result showed: the highest filter paper of the single strain and mixed strains were decomposted 38.92 percent (?) and 49.76 percent(cooperation:B3F2) respectively. Most filter paper lost of mixed strains was higher than single strain. Synergism existed between the strains.The orthogonal experiment showed that the optimum conditions of producing cellulase: the proportion of straw to wheat bran was 7:3, 2 percent peptone (2 percent (NH4)2SO4 in the mid-temperature solid state fermentation), the rate between material and water was 1:1.5.Under the above condition, the highest activity of single strain amounted to 990U/g (Bae) dried koji. The same temperature type of mixed strains amounted to 1693U/g (cooperation: 83583683) dried koji. Including fungi, bacteria and actinomyces, the different temperature type strains of mixed culture were researched preliminarily. CMC enzyme activity for group was the highest that could amount to 2592U/g (cooperation: B3F2F4B35B36S3) dried koji as well as stable. Through co-fermentation of mixed strains, . combination strains of high activity were obtained. The experiment provided many important basis of the research on multi-strain co-fermentation technology and reproductive usage of cellulose resource.
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