Research On Modeling And Application Of Fungal Growth On Interface

This article mainly describes the growth characteristic of fungi on interface. The growths of microorganism on solid-gas interface through establishing simulated systems are studied in the article Rhizopus. oryzae was used for the example throughout the experiment.Firstly, In simulated system of solid-gas interface in which the cellophane paper is laid on the liquid medium, the maximum biomass (Xmax) and maximum specific rate( u max) of Rhizopus. Oryzae was determined on distinct interface materials. Then, the factors that affect the growth of R.oryzae are determined respectively, including the category of medium, concentration and culture conditions. The result proves: the proportion of 12%glucose is good carbon source because it can be absorbed directly. The proportion of 0.4% (NH4) 2864 benefit growth of microorganism, but it is not the best suitable nitrogen source .The enrolling of electric field can not obviously change biomass and the inoculation produce unimportant effect on the maximum biomass which can change the specific growth rate however. The biomass has maximum quantity at 35癈.Secondly, the active zone and inactive zone of microbe is selected in the article, the two-phase model is established which considered the micro and macro growth phenomena all. It is proved that the two-phase model fitted to the growth of microbe on the interface more sufficiently. So, it is useful for the study of microorganism on the interface, which have some limitation, however.Lastly, the simulated system is used for the fermentation of L-lactic acid, which owns the advantages of liquid fermentation and solid fermentation, and overcomes the shortcomings of theirs. The condition of fermentation is determined. Moreover, the cycle-batch system is developed which alleviated the concentration differentiation, and it is worth investigating as a novel culture method.However, how to affect the metabolism of microorganism, effect of electric field extension, and the improvement of reactor is required to study further.