Characteristic And Regulation Of Cellulose Fermentation Using Microbial Mixture MC1

This thesis analyses dynamics of main parameters such as during the fermentation of cellulose by determining the pH value, dissolved oxygen and the dynamics of cellulose enzyme activity etc., in order to determine conditions of solution cellulose degradation fermentation by using MC1 microbial mixture in large scale. A primitive study was conducted about the optimum adding method of cellulose substrate and the optimum fermentation control condition. When pH value curve are concave to the downside the fermentation activity of microbial mixture are higher, on the contrary, if the curve was concave to the upside the activity was restrained. At the same time, the results showed that the optimum ratio of liquid area to depth of container were between 7.9 and 13.5 under static fermentation. Compared to the potential capacity of cellulose decomposition between continuous fermentation and fed-batch culture method. The result show that cellulose decomposition capacity of fed-batch culture fermentation maintains more than 2 months and total decomposed quantity are higher than no fed-batch culture treatment. The decomposed quantity of fed-batch culture treatment was more than 3 g per 100ml liquid medium. By gas chromatography-mass spectrum method the qualitative analysis was conducted for the metabolic production of fermentation using crude cellulose substrate (rice straw). The main metabolic production are formic acid, acetic acid, ethyl acetate, ethanol and lactic acid. At the same time, the dynamics of the yield of main metabolic production were determined. Peak time of metabolic production was known and maximum metabolic production is ethyl acetate, compared to other material during the fermentation. The research provides a short cut to reuse organic waste materials, and improves economic value of a large number of crude cellulose in nature. The results show that MC1 mixture resisted to some plant pathogenous fungi, and it did not show effect on nitrogen fixation and phosphorus dissolving capacity.