Electrochemical Study On Protein And Acceptor Interaction

The quantitative determination of protein is a considerable factor in biochemical and clinical assays. Over recent years, electrochemical methods have been widely used in bioanalytical chemistry. The polarographic wave of protein mainly includes the reduction wave, the catalytic hydrogen wave and the parallel catalytic wave. Recently, a new kinetic wave of catalytic hydrogen wave named as the parallel catalytic hydrogen wave of protein is observed in ammoniac medium containing a suitable oxidant, which is more sensitive than that of the corresponding catalytic hydrogen wave.This paper studies the parallel catalytic hydrogen wave of hemolysin. A direct evaluation and homogeneous voltammetric immunoassay of anti-HSA are proposed to develop more sensitive methods for quick evaluating antibody, respectively. A study on the interaction of chrome-azurol S (CAS) with human serum albumin (HSA) is also investigated. The proposed method is applied to the determination of urine samples with satisfactory results. The binding number and the binding interaction mechanism are also discussed.