Primary Studies On Degradation Of Environmental Pollutants By Laccase From Coriolus Versicolor And Coriolus Versicolor

Degradation of environmental pollutants were studied by using white rot fungus Coriolus versicolor.The results showed that Coriolus versicolor can degrade Benzynine.At 50mg/L,46.83% of Benzynine was degraded.but at 500mg/L,only 10.95% of Benzynine was degraded.Coriolus versicolor cannot degrade Benzidine.Coriolus versicolor can degrade Nitrobenzene,At500mg/L Nitrobenzene concentration,27.603% of Nitrobenzene was degraded. 100mg/L,only33.09%Nitrobenzene was degraded. Low concentration Cresol Mixed Isomers can be degraded by C.versicolor effectively. 100mg/L, 74.8%Cei sol Mixed Isomers was degraded.but,at high concentration(l 500mg/L), 12.13%Cersol Mixed Isomers was degraded.Fermentative Laccase and LacB can degrade Hydroquinone effectively.The reaction process conformed to one-rate dynamic equation. At different concentration of Hydroquinone,pramaters of equations are different,at same concentration of Hydroquinone,ti/2(LacB)>ti/2(fermentative laccase).The study of enzymatic dynamic reaction showed that the reaction conformed to M-M equation.Km and Vmax were 42.1585mg/liter and 1.9716mg/min respectively.In this experiment,1 have studied the kinetic equations of the degradation of m-cresol by Coriolus versicolor and growth of Coriolus versicolor and consumption of starch and Laccase production.At three cases as follows,the growth kitietic equations of C.versicolor comforned to Logistic equations.the equations were:With no m-cresol: Cx(t)=0.172737e0.077563t/(1-8.47 X 10-5( 1-e0.077563t)) With low m-cresol Cx(t)=3.39999e0.037333t/(1-0.0036(1-e0.037333t))With high m-cresol Cx(t)=71.4799e 0.012907t/(1-0.232(1-e0.012907t))Laccase production kinetic equations.At absence of m-cresol and low m-cresol,theequations comforned to one-rate kinetic equations The equations were: At absence ofm-cresol: Yt=84.5722(l-e -0.016483(t-108) At low m-cresol: Yt=120.6284(l-e-0.074499(t-168))At high concentration of m-cresol,the equation of Laccase production conformed to Logistic equation.The equation is Yt=l 54.041 l/(l+e (13-0352-0.034856t))Kinetic equations of starch consumption of C.versicolor conformed to Logistic equations also.They can described:With no m-cresol:C(t)=2144.2870/(l+e(-1.9661+0.015078t)) With low m-cresol:C(t)=1999.5725/(l+e(-1.7426+0.010653t) With high m-cresol:C(t)=1438.2295/(l+e(-24994+0-011821t)) The three equations showed that the consumption of starch can be partially competed by addition ofm-cresol.The kinetic equations of growth of C.versicolor and consumption of starch conformed to Monod equations.The equations were:With no m-cresol:V=0.027588*Cs/(l34.4182+Cs) With low m-cresol:V=0.024643*Cs/(321.3973+Cs) With high m-cresol:V=0.009684*Cs/( 149.3178+Cs)Kinetic equations of m-cresol degradation. At low concentration of m-cresol,lhe equation conformed to Logistic equalion.the equation can be described as C(l)=161.5418/(l W-16,9077+0.077747t)) At high concentration of m-crcsol,the degradation ofm-cresol can be described one-rate kinetic equation.IlnC= -0.002U + 5.6389...