Production Of Itaconic Acid From Corn Starch By Fermentation

Corn is one of the three main crop in the world. The production of China i s at the second p I ace in the world. The total production per year have achieved 0. 12 bi I I ion ton which is in excess of what is needed. The reduction of agricultural surpluses and the uti I ization of marginal and set-aside lands are amongst the main objectives.Itaconic acid , an unsaturated dicarboxylic acid, has been found widespread commerciaI uses. Production of itaconic acid from corn starch by fermentation can not only decrease the deposit of crop, but also increase the integrate strength of chemical industry.This paper includes four parts: screening for a high-productive mutant from corn starch as a carbon source; preparat i on of hydro I ysed cron starch; researching for an opt i ma I condition for fermentation; separation and characteristic research of itaconic acid.A ser ies of treatments has been done to the initial strain Aspergi I I us Terreus to breed a new mutant AS5032 which has high-productivity of itaconic acid and the carbon source of Aspergi I lus terreus is corn starch that was sacchar if i ed at 35% DEbyamylase. Meanwhile, this study carry out a series of experiments on nutrient content , inoculation volume, precultivate time, pH value, aeration. In conelus ion, the production medium contained: corn starch saccharified at 35% DE 10%> NH4NO3 0. 4%, growth accelerant 0. 3%,KH2P04 0. 025%,,MgS04 0.2%,pHvalue 3. 0 . After incubation on a rotary shaker at 200rpm for 108h at 35C, 5.41% itaconic acid was produced by AS5032 in flask fermentation from the medium. The yield of itaconoc acid was higher than 50%.In addition, this study stilltried experiments on the separation of itaconic acid with three kinds of processes.