Microbial Degradation Of Fenoxaprop-p-ethyl

Bacterial strains which could decompose fenoxaprop-p-ethyl were isolated from a batch culture with fenoxaprop-p-ethyl as carbon source and the sludge from waste water dealing system of Huaxing chemical engineering Co L.td . After determining the rest quantity of fenoxaprop-p-ethyl with HPLC , H was selected as dominant degradation bacterial strain for its best degradation ability to fenoxaprop-p-ethyl . H8-1 was also selected as another dominant degradation bacterial strain for its higher degradation ability to fenoxaprop-p-ethyl than the other strains after UV mutation . Fenoxaprop-p-ethyl degradation dynamics was studied with HPLC method , and degradation products of fenoxaprop-p-ethyl had been separated with HPTLC . The main study results are as follows :1 .Eight bacterial strains which could decompose fenoxaprop-p-ethyl were isolated from a batch culture with fenoxaprop-p-ethyl as carbon source and the sludge from waste water dealing system of Huaxing chemical engineering Co L.td . After determining the rest quantity of fenoxaprop-p-ethyl with HPLC , H was selected as dominant degradation bacterial strain for its best degradation ability to fenoxaprop-p-ethyl .Based on the morphological appearances and physiological characteristics ,H was identified as Alcaligenes sp and named as Alcaligenes sp.H .2.H8-1 was also selected as another dominant degradation bacterial strain for its higher degradation ability to fenoxaprop-p-ethyl than the other strains after UV induce . H8-1 was marked as Alcaligenes sp. Huv . With the concentration of fenoxaprop-p-ethyl varing from 5mg/L to 100mg/L , Alcaligenes sp. Huvhas better degradation ability to fenoxaprop-p-ethyl than Alcaligenes sp.H .3. fenoxaprop-p-ethyl degradation dynamics was studied . Degradation kinetics follow the first order kinetics .4. It proved that degradation ability of mixed bacterial by Alcaligenes sp.H and Alcaligenes sp. Huv to fenoxaprop-p-ethyl is superior to Alcaligenes sp.H , superior to Alcaligenes sp. Huv after the quantity of Alcaligenes sp. Huv surpasses half , and with the quantity increasing of Alcaligenes sp. Huv , degradation ability of mixed bacterial by and Alcaligenes sp. Huv to fenoxaprop-p-ethyl increases respectively comparing their half-time . Their half-times are 37.66h, 30.39h and 23.90h to mixed bacterial (Alcaligenes sp.H : Alcaligenes sp. Huv 2.5:1,1:1,1:2.5 ) ,64.17h to Alcaligenes sp.H and 35.18h to Alcaligenes sp. Huv.5. Degradation rates of fenoxaprop-p-ethyl were positively correlative to biomass .On thecontrary , the fortified concentration is opposite the degradative rate . It can be seen from their half-time and their half-time are 67.28h, 35.0h and 21.72h to Alcaligenes sp.H , 35.54h ,21.32h and 19.58h to Alcaligenes sp. Huv correlative to their different biomass .Results showed that biomass has a appropriately fortified concentration .When surpassing this biomass , the degradative rate can not grow obviously .6. With the concentration of fenoxaprop-p-ethyl varing from 5mg/L to 100mg/L , the half-time of Alcaligenes sp.H and Alcaligenes sp. Huv increased respectively .The different half-time of Alcaligenes sp.H and Alcaligenes sp. Huv with different concentration are 47.47h,65.38h,68.61h,86.63h,135.88h and 18.24h,35.54h,46.51h,57.27h,72.19h .Effects of different concentration of fenoxaprop-p-ethyl to degradation showed that Alcaligenes sp.H and Alcaligenes sp. Huv had better degradation in some a concentration and the degradation ability to fenoxaprop-p-ethyl of Alcaligenes sp.H and Alcaligenes sp. Huv was obviously decreased in high concentration.7. Chlorothalonil, dodecylbenfenxaprop -p-ethylene sulfonic aid sodium salt and Cr+6can inhibite the degradation ability of Alcaligenes sp.H , Alcaligenes sp. Huv and the mixed bacterial by Alcaligenes sp.H and Alcaligenes sp. Huv to fenoxaprop-p-ethyl. The degradation ability of Alcaligenes sp.H to fenoxaprop-p-ethyl was severely inhibited by Chlorothalonil. In early period , Alcaligenes sp.H , Alcaligenes sp. an...