Research Of Piezoelectric Sensitive & Molecular Imprinting

Piezoelectric biosensor transfers the mass change of the substance absorbed on the electrode of the sensor into the change of the frequency via the Piezoelectric effect of the quartz crystal. It has many advantages such as high sensitivity and needs no marks；molecular imprinting is an efficient way to prepare simulative antibody, acceptor by synthesis method。This thesis mainly was concerned with the method to fix the protein on the electrode of the Piezoelectric biosensor,the reaction of the immobilized enzyme and Organophosphate and the in-situ synthesis of molecularly imprinted polymers。As for the method to fix up the protein on the electrode,this thesis discussed sequentially the absorption of the Bovine Scrum Albumin(BSA) on the electrode of the sensor and the relations between the absorption of BSA and the pH as well as the concentration of BSA；proper conditions for the cross-fixation of the Bovine Scrum Albumin using the Glutaraldehyde and the cross-fixation of the Acetylcholine Esterase (AChE) using Glutaraldehyde,Bovine Scrum Albumin。Results showed that the pH of the solution had a great influence on the absorption of the Bovine Scrum Albumin on the electrode of the sensor, when pH=7,the absorption of the Bovine Scrum Albumin on the electrode met Langmuir`s isothermal absorption equation；when the concentration of Glutaraldehyde was kept at a constant, the Bovine Scrum Albumin fixed on the electrode gradually increased along with the increase of the concentration of the solution；proper condition for the fixation of the Acetylcholine Esterase was that: Glutaraldehyde 10%,Bovine Scrum Albumin 0.1mg/mL,Acetylcholine Esterase 0.1mg/mL.The thesis also discussed the absorption kinetics of the Bovine Scrum Albumin on the gold electrode of the Piezoelectric biosensor.The thesis investigated on the interaction between Organophosphorus compound—Trichlorfon and Acetylcholine Esterase which fixed on the gold electrode of the sensor. According to the frequency-time curve of the solution with different concentrations,along with kinetics analysis,Results showed that within the experimental range of the concentration, the rate of the reaction and the concentration displayed a linear relationship,as for Trichlorfon,the reaction was the first order reaction process, the reactive rate constant K0=5.658×106m3(s-2(kg-1。The later simulated the experience formulae, According to the frequency-time curve of the reaction process and the rate-time relationship of the reaction. After treating the experimental data with the experience formulae above,Results showed that the initial reaction rate and the concentration of the solution of the Trichlorfon followedthe linear relationship, the reactive rate constant k=3.88×106m3(s-2(kg-1.As for the in-situ synthesis of the molecularly imprinted polymers . It was discussed that the relation of the ratio of template molecular (Chloramphenicol) to the functional monomer ( Methacrylic Acid 2-Diethylaminoethyl Ester) and the synthesis of Chloramphenicol molecularly imprinted polymers. The conclusion was drawn that the rate of molecular imprinting reaction was proportion to the square of the functional monomer's concentration.Eventually, the thesis discussed the developing trend of the Piezoelectric biosensor and the technology of the molecular imprinting.