A Fundamental Study On Biodegradation And Bioremediation Of The Oil-contaminated Area Of Harbor

This paper studied on bioremediation of the oil-contaminated area of harbor and analyzed the basic principle of the technology of bioremediation.The technology of bioremediation is considered to be a preferred bacterial treatment with the oil-contaminated area of harbor in contrast with other treatments. It is necessary to study aerobic treatment with in-situ bioremediation by means of inoculated microorganism and added nutrition and co-metabolism substrate. This paper also discussed the application of oil dispersant agent and bio-surfactant agent in oil-contaminated bioremediation. The result of test shows that it is feasible to cultivate salt-resistant activated-sludge microorganism as the inoculation of bioremediation. The experiment of respiration reveals the great differences in the biochemical property of some dominant pollutant of oil. The order of biochemical property is toluene better than m-xylene, m-xylene better than benzene, benzene better than p-xylene, p-xylene better than ethylbenzene and ethylbenzene better than O-xylene. The result of shaking culture test indicated that the oil can be degraded by the activated sludge culture in short time between 5 and 15 mg/L. The rule of degradation follows the first-order kinetic reaction equation. On the basis of the test, suitable operating conditions are recommended as follows, pH=6.5,T=35℃,the shaking rate=120 r min-1, quantity of inoculation=30%. The experimental results show that the coexist of easily biodegradable yeast cream is favorable for the biodegradation of oil. The increase of ratio of biodegradation with the concentration of yeast cream added follows the Michaelia-Menten equation.