The Separation And The Studying Of Degradation Ability Of Coke-Plant Wastewater Preponderant Bacterium

The components of coke-plant wastewater are very complex. There are large amounts of refractory organics in it, and their concentrations are high. At present, the removal rate of the refractory organics by the conventional activated sludge process is low, which results in a relatively high value of COD in effluent, so the quality of effluent cannot reach the national draining standard. So, recently, the scientific researchers precede extensive research, and then look for the high-efficiency, economy ways to treat coke-plant wastewater, which improve environment quality and realize water-resource recycle.This paper has recounted the origin, characteristic, mostly constituting, actuality and advance in the technology of treating coke-plant wastewater. Among the treatment of toxic organic wastewater, bioaugmentation has been discussed, and applied in coke-plant wastewater treatment. Therefore, Separate the bacterium strains from the environment, study the bacterium strains' degradation capability in treating coke-plant wastewater to provide the good bacterium strains for biodegradation of actual wastewater.Effective strains were isolated from coke-plant wastewater and sludge cultivated by coke-plant wastewater, then separated and identified simply. Detailed study has been carried out on the separated efficient strains' degradation capability, the influence of their biodegradation by primary carbon for co-metabolizing , inorganic ion, the removal effect on coke-plant wastewater according to COD data. Seven effective bacterium strains was separated: JB1, JB2, SB3, JB4 , JB5 , JB6, JB7. The identification result is that JB1 belongs to Pseudomonaceae JB2 belongs to Neisseriaceae, JB3, JB4 belongs to Flavobacterium, JB5 , JB7 belongs to Corynebacteriaceae, JB6 belongs to Bacillaceae.Detailed study had been carried out on specific property of the separated efficient bacteria. The experimental research result show that it is the best cultivate condition ,when temperature is 30 C, pH is 7,shaking culture is at 110r/min. All these conditions are favorable for the growth and reproduction of the strains. Primary carbon and nitrogen source , inorganic ion can effectively accelerated the degradation processes of coke plant wastewater.The orthogonal experimental research result show that the removal ratio can be speeded up, when adding 10mg/L manganese ion, 0.5 mg/L ferrous ion, 0.5 mg/L copper ion, and 10mg/L calcium ion into the culture media which including coke-plant wastewater. Under the synthesis condition ,the result is that the degradation effect is obvious, when adding 4g/L glucose with corresponding inorganic ion, JB1: JB4=1: 2 the proportion of bacteria, and 30mL culture media.The results of application studying show that effective strains can obviously raise COD removing rate of coke-plant wastewater cooperating with activated sludge. It will be possible that effluent COD meet with the national draining standard when the isolates are utilized in treatment system. The experimental results obtained in this paper provide references for further research and utilization.