Dense Pellicular Magnetic Agarose Media For Protein Adsorption By Magnetically Stabilized Fluidized Bed

The magnetically stabilized fluidized bed uses an externally applied, axially ortransversely aligned magnetic field to independently control the magnetic particlesmovement in the bed. Even at high liquid velocities, magnetic stabilization can serveto suppress mixing of the solid particles, thus, MSFB exhibit a combined advantageof packed bed and fluidized bed in a liquid fluidized bed system. In this article, dense pellicular magnetic supports for magnetically stabilizedfluidized bed adsorption of protein have been manufactured, with the introduction ofmagnetic colloid particles and glassbead during the emulsification of melted 4%agarose. After screened with standard sieves the support's diameter ranges from 72to 130 μm, and volume-weighted mean diameter is 100 μm. The small particlediameter combined with its pellicular structure make the diffusion path length veryshort. The mean density of the supports is 1.40±0.03 g/mL. The supports have goodmagnetization property with little residual magnetization after the field was removed.After it has been cross-linked and reduced for further use in adsorption of protein,these magnetic supports were coupled with diethylaminoethyl (DEAE). Itsadsorption characteristics were evaluated, using BSA as a model protein to test theadsorption capacity and kinetics. Fluidization and stabilization behavior of thesesupports were examined in a small-scale magnetically stabilized fluidized bedcomparing with in expanded bed. Breakthrough curves of BSA in MSFB and DBCof these DEAE-g-Fe-a supports were also examined. The results indicate that thissupport is promising for MSFB chromatography.