Identification Of Microorganisms Isolated From Apples And Effects Of High-voltage Pulse Magnetic Field On These Microorganisms

The API identification system in combination with classical isolation methods was used as an original approach to determine the strains of microbes from apples. The main aim of this research paper was to learn about the microorganisms in apples and to help controlling the microbes during fruit juice processing. Furthermore the physiological states of damaged cells of Saccharomyces cerevisiae, Escherichiae coli, Salmonella sp. and Staphylococcus aureus after pulsed high magnetic field were investigated.The number of total clones in apples was found to range from 105 to 107 cfu/g. The identification of strains isolated by API system revealed 25 yeast species representing 6 genera: Candida krusei (13), Saccharomyces cerevisiae (5), Kloeckera spp. (2), Candida magnoliae (2), Candida tropicalis (1) ,Candida kefyr (1), the accuracy of identification was >95%. 6 bacillus strains: Bacillus subtilis (4), Bacillus licheniformis (2), the accuracy of identification was >90% and 10 lactobacillus strains: Lactobacillus plantanan (6), Lactobacillus acidophilus (2), Lactobacillus brevis (1), the accuracy of identification was >85%.Scanning electronmicroscopy (SEM) revealed that the E.coli, S.cerevisiae, Salmonella and S.aureus cells treated by pulsed high magnetic field for 4.2 T and 30 pulses some physical damages were inflicted and flow cytometry (FCM) revealed that the damage cells' membrane integrity was damaged. The emission of treated cells was S.aureus>Salmonella>E.coli> S.cerevisiae. The SEM and FCM demostrated that the center point of treated cells by pulsed high magnetic field was membrane.